Genomic characterization of the italian wolf (Canis lupus): the genes involved in black coat colour determination and application of microarray technique for snps detection.

Greco, Claudia (2009) Genomic characterization of the italian wolf (Canis lupus): the genes involved in black coat colour determination and application of microarray technique for snps detection. , [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Biodiversità ed evoluzione, 21 Ciclo. DOI 10.6092/unibo/amsdottorato/1729.
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Abstract

This study provides a comprehensive genetic overview on the endangered Italian wolf population. In particular, it focuses on two research lines. On one hand, we focalised on melanism in wolf in order to isolate a mutation related with black coat colour in canids. With several reported black individuals (an exception at European level), the Italian wolf population constituted a challenging research field posing many unanswered questions. As found in North American wolf, we reported that melanism in the Italian population is caused by a different melanocortin pathway component, the K locus, in which a beta-defensin protein acts as an alternative ligand for the Mc1r. This research project was conducted in collaboration with Prof. Gregory Barsh, Department of Genetics and Paediatrics, Stanford University. On the other hand, we performed analysis on a high number of SNPs thanks to a customized Canine microarray useful to integrate or substitute the STR markers for genotyping individuals and detecting wolf-dog hybrids. Thanks to DNA microchip technology, we obtained an impressive amount of genetic data which provides a solid base for future functional genomic studies. This study was undertaken in collaboration with Prof. Robert K. Wayne, Department of Ecology and Evolutionary Biology, University of California, Los Angeles (UCLA).

Abstract
Tipologia del documento
Tesi di dottorato
Autore
Greco, Claudia
Supervisore
Dottorato di ricerca
Scuola di dottorato
Scienze biologiche, biomediche e biotecnologiche
Ciclo
21
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
melanism, black phenotype, coat colour, K locus, SNPs, microarray, Italian wolf.
URN:NBN
DOI
10.6092/unibo/amsdottorato/1729
Data di discussione
18 Maggio 2009
URI

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