Fatty acids containing lipids and membrane lipidomics in regenerative medicine and nutraceutical applications

Lipidomics is a powerful tool for studying dynamic lipid changes in both physiological and pathological conditions. Lipids and fatty acids are gaining increasing interest across multiple disciplines, from chemistry and biochemistry to nanobiotechnology, particularly in lipid-based formulations and membrane studies. This Industrial PhD thesis, supported by Silfradent, explores lipidomic applications in fatty acids chemistry, biological sample analysis, regenerative medicine, and nutraceuticals. Chapter 1 investigates the impact of radical stress on membrane phospholipids, focusing on Plasmalogens, a unique class of phospholipids. We demonstrated, for the first time, cis-trans isomerization of plasmalogens under thiyl radical stress, affecting membrane supramolecular organization and potentially contributing to disease mechanisms. Chapter 2 focuses on analytical methodologies for lipid profiling. We highlight how acidic conditions alter plasmalogens and PUFA-containing lipids, affecting data reliability. Our findings emphasize the need for optimized protocols in lipidomic studies. Chapter 3 applies lipidomics to regenerative medicine, investigating PRP lipid composition and its role in fibroblast membrane remodeling. While PRP is mainly studied for growth factors, our results reveal its lipid-driven regenerative potential. This research was developed within Silfradent’s PRP and regenerative medicine framework. Chapter 4 explores lipid-based formulations, developing O/W emulsions and lactoferrin nanoparticles for nutraceutical and pharmaceutical applications. We demonstrate that lecithin enhances GLA membrane incorporation from Borage oil, promoting anti-inflammatory membrane remodeling in healthy and tumor cell lines. Additionally, Lactoferrin nanoparticles stabilize Evening Primrose Extract, supporting advanced delivery systems. This research was partially conducted at the Medical University of Lodz, Poland. This PhD work lays a foundation for future advancements in lipidomics, regenerative medicine, and nutraceutical science, fostering cross-disciplinary innovation and applications.