From "wet biology" to statistical analysis of structural features with bioinformatics tools

Marani, Paola (2008) From "wet biology" to statistical analysis of structural features with bioinformatics tools, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Biotecnologie cellulari e molecolari, 20 Ciclo. DOI 10.6092/unibo/amsdottorato/689.
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Abstract

Many new Escherichia coli outer membrane proteins have recently been identified by proteomics techniques. However, poorly expressed proteins and proteins expressed only under certain conditions may escape detection when wild-type cells are grown under standard conditions. Here, we have taken a complementary approach where candidate outer membrane proteins have been identified by bioinformatics prediction, cloned and overexpressed, and finally localized by cell fractionation experiments. Out of eight predicted outer membrane proteins, we have confirmed the outer membrane localization for five—YftM, YaiO, YfaZ, CsgF, and YliI—and also provide preliminary data indicating that a sixth—YfaL—may be an outer membrane autotransporter.

Abstract
Tipologia del documento
Tesi di dottorato
Autore
Marani, Paola
Supervisore
Dottorato di ricerca
Ciclo
20
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
outer membrane protein bioinformatics secb autotransporter
URN:NBN
DOI
10.6092/unibo/amsdottorato/689
Data di discussione
6 Giugno 2008
URI

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