Molecular diversity of Primula apennina and phylogeny of Primula Subsection Euauricula

Crema, Silvia (2009) Molecular diversity of Primula apennina and phylogeny of Primula Subsection Euauricula, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Biodiversità ed evoluzione, 21 Ciclo.
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Abstract

Primula apennina Widmer is endemic to the North Apennines (Italy). ISSR were used to detect the genetic diversity within and among six populations representative of the species distribution range. High levels of genetic diversity were revealed both at population (PPB = 75.92%, HS = 0.204, Hpop = 0.319) and at species level (PPB = 96.95%, HT = 0.242, Hsp = 0.381). Nei gene diversity statistics (15.7%), Shannon diversity index (16.3%) and AMOVA (14%) detected a moderate level of interpopulation diversity. Principal coordinate and bayesian analyses clustered the populations in three major groups along a geographic gradient. The correlation between genetic and geographic distances was positive (Mantel test, r = 0.232). All together, these analyses revealed a weak but significant spatial genetic structure in P. apennina, with gene flow acting as a homogenizing force that prevents a stronger differentiation of populations. Conservation measures are suggested based on the observed pattern of genetic variability. P. apennina belongs to Primula subsect. Euauricula which includes 15 species distributed on the whole Alps and Apennines. A phylogenetic analysis was carried out using AFLP markers in order both to clarify the relationships among the species of subsection Euauricula that remained unresolved in previous works and to make some hypoteses on their evolutive dynamics. NJ, PCO and BAPS analyses strongly confirmed the monophyly of P. subsect. Euauricula and all the species form strongly supported clades. NJ tree topology suggested a simultaneous fragmentations of ancestral species in a large number of isolated populations that survived in refugia along the unglaciated margins of the Alps in response to the Pleistocene climatic oscillations.

Abstract
Tipologia del documento
Tesi di dottorato
Autore
Crema, Silvia
Supervisore
Dottorato di ricerca
Scuola di dottorato
Scienze biologiche, biomediche e biotecnologiche
Ciclo
21
Coordinatore
Settore disciplinare
Parole chiave
P. apennina, Primula subsect. Euauricula, gene diversity, phylogeny, AFLPs, ISSRs
URN:NBN
Data di discussione
18 Maggio 2009
URI

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