Sviluppo di metodiche innovative per la diagnosi e lo studio di malattie ad eziologia virale dei pesci

De Aguiar Saldanha Pinheiro, Ana Cristina (2015) Sviluppo di metodiche innovative per la diagnosi e lo studio di malattie ad eziologia virale dei pesci, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6939.
Documenti full-text disponibili:
[img]
Anteprima
Documento PDF (English) - Richiede un lettore di PDF come Xpdf o Adobe Acrobat Reader
Download (2MB) | Anteprima

Abstract

The increase in aquaculture operations worldwide has provided new opportunities for the transmission of aquatic viruses. The occurrence of viral diseases remains a significant limiting factor in aquaculture production and for the sustainability. The ability to identify quickly the presence/absence of a pathogenic organism in fish would have significant advantages for the aquaculture systems. Several molecular methods have found successful application in fish pathology both for confirmatory diagnosis of overt diseases and for detection of asymptomatic infections. However, a lot of different variants occur among fish host species and virus strains and consequently specific methods need to be developed and optimized for each pathogen and often also for each host species. The first chapter of this PhD thesis presents a complete description of the major viruses that infect fish and provides a relevant information regarding the most common methods and emerging technologies for the molecular diagnosis of viral diseases of fish. The development and application of a real time PCR assay for the detection and quantification of lymphocystivirus was described in the second chapter. It showed to be highly sensitive, specific, reproducible and versatile for the detection and quantitation of lymphocystivirus. The use of this technique can find multiple application such as asymptomatic carrier detection or pathogenesis studies of different LCDV strains. The third chapter, a multiplex RT-PCR (mRT-PCR) assay was developed for the simultaneous detection of viral haemorrhagic septicaemia (VHS), infectious haematopoietic necrosis (IHN), infectious pancreatic necrosis (IPN) and sleeping disease (SD) in a single assay. This method was able to efficiently detect the viral RNA in tissue samples, showing the presence of single infections and co-infections in rainbow trout samples. The mRT-PCR method was revealed to be an accurate and fast method to support traditional diagnostic techniques in the diagnosis of major viral diseases of rainbow trout.

Abstract
Tipologia del documento
Tesi di dottorato
Autore
De Aguiar Saldanha Pinheiro, Ana Cristina
Supervisore
Dottorato di ricerca
Scuola di dottorato
Scienze veterinarie
Ciclo
27
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
Molecular Diagnostic Techniques,fish viruses,Real-time PCR,LCDV,Multiplex RT-PCR,VHSV,IHNV,IPNV and SDV.
URN:NBN
DOI
10.6092/unibo/amsdottorato/6939
Data di discussione
17 Aprile 2015
URI

Altri metadati

Statistica sui download

Gestione del documento: Visualizza la tesi

^