Molecular strategies for genetic diversity analysis and development of markers linked to resistance traits in apple

Liang, Wei (2013) Molecular strategies for genetic diversity analysis and development of markers linked to resistance traits in apple , [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Colture arboree ed agrosistemi forestali ornamentali e paesaggistici, 25 Ciclo. DOI 10.6092/unibo/amsdottorato/5910.
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Abstract

The goal of many plant scientists’ research is to explain natural phenotypic variation in term of simple changes in DNA sequence. DNA-based molecular markers are extensively used for the construction of genome-wide molecular maps and to perform genetic analysis for simple and complex traits. The PhD thesis was divided into two main research lines according to the different approaches adopted. The first research line is to analyze the genetic diversity in an Italian apple germplasm collection for the identification of markers tightly linked to targeted genes by an association genetic method. This made it possible to identify synomym and homonym accessions and triploids. The fruit red skin color trait has been used to test the reliability of the genetic approaches in this species. The second line is related to the development of molecular markers closely linked to the Rvi13 and Rvi5 scab resistance genes, previously mapped on apple’s chromosome 10 and 17 respectively by using the traditional linkage mapping method. Both region have been fine-mapped with various type of markers that could be used for marker-assisted selection in future breeding programs and to isolate the two resistance genes.

Abstract
Tipologia del documento
Tesi di dottorato
Autore
Liang, Wei
Supervisore
Co-supervisore
Dottorato di ricerca
Scuola di dottorato
Scienze agrarie
Ciclo
25
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
apple, genetic diversity, scab resistance, fine-mapping, molecular markers
URN:NBN
DOI
10.6092/unibo/amsdottorato/5910
Data di discussione
9 Aprile 2013
URI

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