Study of Venturia inaequalis sensitivity to fungicides through molecular and biological methodologies

Turan, Ceren (2013) Study of Venturia inaequalis sensitivity to fungicides through molecular and biological methodologies, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Ecologia microbica e patologia vegetale, 25 Ciclo. DOI 10.6092/unibo/amsdottorato/5638.
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This study was aimed to correlate the results of relative germination from in vitro tests by trifloxystrobin with those of qPCR on a wide range of V. inaequalis populations and monoconidial isolates. Samples were collected in Italian and Turkish distinct locations from orchards with different scab management. In this study, an allele-specific qPCR with primer sets designed was successfully developed to quantitatively determine the frequency of QoI-resistant allele G143A in populations and monoconidial isolates of V. inaequalis. qPCR followed a similar pattern to that obtained using in vitro conidial germination test in very sensitive and very resistant populations. However, the variability between two test results was observed in hetereogenous populations. Therefore, the results of correlations between in vitro and qPCR showed a positive but not very high correlation for Venturia inaequalis populations (R2=0.70). On the contrary, this correlation between two assessment methods was very high for monoconidial isolates (R2=0.92). Results obtained in quantitative PCR and from traditional spore germination assay differed for the same fungal population and in some cases, it is difficult to assess the resistance in the field by only qPCR. It was concluded that it is not always possible to correlate the frequency of detection of the mutation with biological assessment. In such situations, monitoring by molecular techniques must be supported by standard in vitro resistance assessments and observation of field performance in order to have correct conclusions.

Tipologia del documento
Tesi di dottorato
Turan, Ceren
Dottorato di ricerca
Scuola di dottorato
Scienze agrarie
Settore disciplinare
Settore concorsuale
Parole chiave
venturia inaequalis, qPCR , strobilurin, cytochrome b
Data di discussione
11 Aprile 2013

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