Molecular mechanisms involved in the pathogenesis of beet soil-borne viruses.

Delbianco, Alice (2013) Molecular mechanisms involved in the pathogenesis of beet soil-borne viruses., [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Ecologia microbica e patologia vegetale, 25 Ciclo. DOI 10.6092/unibo/amsdottorato/5353.
Documenti full-text disponibili:
Documento PDF (English) - Richiede un lettore di PDF come Xpdf o Adobe Acrobat Reader
Download (8MB) | Anteprima


The genus Benyvirus includes the most important and widespread sugar beet viruses transmitted through the soil by the plasmodiophorid Polymyxa betae. In particular Beet necrotic yellow vein virus (BNYVV), the leading infectious agent that affects sugar beet, causes an abnormal rootlet proliferation known as rhizomania. Beet soil-borne mosaic virus (BSBMV) is widely distributed in the United States and, up to date has not been reported in others countries. My PhD project aims to investigate molecular interactions between BNYVV and BSBMV and the mechanisms involved in the pathogenesis of these viruses. BNYVV full-length infectious cDNA clones were available as well as full-length cDNA clones of BSBMV RNA-1, -2, -3 and -4. Handling of these cDNA clones in order to produce in vitro infectious transcripts need sensitive and expensive steps, so I developed agroclones of BNYVV and BSBMV RNAs, as well as viral replicons allowing the expression of different proteins. Chenopodium quinoa and Nicotiana benthamiana plants have been infected with in vitro transcripts and agroclones to investigate the interaction between BNYVV and BSBMV RNA-1 and -2 and the behavior of artificial viral chimeras. Simultaneously I characterized BSBMV p14 and demonstrated that it is a suppressor of post-transcriptional gene silencing sharing common features with BNYVV p14.

Tipologia del documento
Tesi di dottorato
Delbianco, Alice
Dottorato di ricerca
Scuola di dottorato
Scienze agrarie
Settore disciplinare
Settore concorsuale
Parole chiave
benyvirus silencing chimeras agroinfection
Data di discussione
11 Aprile 2013

Altri metadati

Statistica sui download

Gestione del documento: Visualizza la tesi