Di Nunzio, Mattia
(2009)
N-3 fatty acids and cardiovascular prevention, [Dissertation thesis], Alma Mater Studiorum Università di Bologna.
Dottorato di ricerca in
Scienze degli alimenti, 21 Ciclo.
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Abstract
In this study we elucidate the role of polyunsaturated fatty acids (PUFAs) in the prevention of cardiovascular diseases, focusing the attention on their role in the
modulation of acyl composition of cell lipids and of gene expression. Regarding this latter mechanism, the effectiveness of PUFAs as activators of two transcriptional factors, SREBPs and PPARs, have been considered. Two different model system have been used: primary cultures of neonatal rat cardiomyocytes and an human hepatoma cell line (HepG2). Cells have been supplemented with different PUFAs at physiological concentration, and special attention
has been devoted to the main n-3 PUFAs, EPA and DHA.
PUFAs influence on global gene expression in cardiomyocytes has been evaluated using microarray technique. Furthermore, since it is not fully elucidated which transcription factors are involved in this modulation in the heart, expression and activation of the three different PPAR isoforms have been investigated. Hepatocytes have been used as experimental model system in the evaluation of PUFAs effect on SREBP activity. SREBPs are considered the main regulator of cholesterol and triglyceride synthesis, which occur mainly in the liver. In both experimental models the modification of cell lipid fatty acid composition subsequent to PUFAs supplementation has been evaluated, and related to the effects observed at molecular level. The global vision given by the obtained results may be important for addressing new researches and be useful to educators and policy makers in setting recommendations for reaching optimal health through good nutrition.
Abstract
In this study we elucidate the role of polyunsaturated fatty acids (PUFAs) in the prevention of cardiovascular diseases, focusing the attention on their role in the
modulation of acyl composition of cell lipids and of gene expression. Regarding this latter mechanism, the effectiveness of PUFAs as activators of two transcriptional factors, SREBPs and PPARs, have been considered. Two different model system have been used: primary cultures of neonatal rat cardiomyocytes and an human hepatoma cell line (HepG2). Cells have been supplemented with different PUFAs at physiological concentration, and special attention
has been devoted to the main n-3 PUFAs, EPA and DHA.
PUFAs influence on global gene expression in cardiomyocytes has been evaluated using microarray technique. Furthermore, since it is not fully elucidated which transcription factors are involved in this modulation in the heart, expression and activation of the three different PPAR isoforms have been investigated. Hepatocytes have been used as experimental model system in the evaluation of PUFAs effect on SREBP activity. SREBPs are considered the main regulator of cholesterol and triglyceride synthesis, which occur mainly in the liver. In both experimental models the modification of cell lipid fatty acid composition subsequent to PUFAs supplementation has been evaluated, and related to the effects observed at molecular level. The global vision given by the obtained results may be important for addressing new researches and be useful to educators and policy makers in setting recommendations for reaching optimal health through good nutrition.
Tipologia del documento
Tesi di dottorato
Autore
Di Nunzio, Mattia
Supervisore
Dottorato di ricerca
Scuola di dottorato
Scienze agrarie
Ciclo
21
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
N-3 fatty acids cardivascular prevention
URN:NBN
Data di discussione
27 Maggio 2009
URI
Altri metadati
Tipologia del documento
Tesi di dottorato
Autore
Di Nunzio, Mattia
Supervisore
Dottorato di ricerca
Scuola di dottorato
Scienze agrarie
Ciclo
21
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
N-3 fatty acids cardivascular prevention
URN:NBN
Data di discussione
27 Maggio 2009
URI
Gestione del documento: