Engineering compartmentalized systems at the nanoscale

This work is focused on investigating compartmentalization effects on the operation of molecules and molecular machines in artificial systems. The thesis is divided into three experimental chapters encompassing different approaches to environmental compartmentalization and their subsequent influence provided to the system components, ranging on different complexity scales from phase-phase separation to molecular confinement. In the first project, it is described how a compartmentalized environment can affect the operation of molecular switches. The second project focused on approaching the opportunity of liposome membrane functionalization. In the third project, the effects of molecular crowding on the internal rotational processes in enamines were studied.