Metabolomics investigations towards formulated natural complex products by untargeted and targeted mass spectrometry-based approaches

Tamimi, Sara (2018) Metabolomics investigations towards formulated natural complex products by untargeted and targeted mass spectrometry-based approaches, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Chimica, 30 Ciclo. DOI 10.6092/unibo/amsdottorato/8559.
Documenti full-text disponibili:
[img]
Anteprima
Documento PDF (English) - Richiede un lettore di PDF come Xpdf o Adobe Acrobat Reader
Disponibile con Licenza: Salvo eventuali più ampie autorizzazioni dell'autore, la tesi può essere liberamente consultata e può essere effettuato il salvataggio e la stampa di una copia per fini strettamente personali di studio, di ricerca e di insegnamento, con espresso divieto di qualunque utilizzo direttamente o indirettamente commerciale. Ogni altro diritto sul materiale è riservato.
Download (2MB) | Anteprima

Abstract

Although the last decade has witnessed a marked growth in the market of plant-based natural products, their high complexity in terms of composition makes a challenging task the guarantee of quality, efficacy and safety requirements. In such scenario, the recently approved European regulation EU 2017/745 has created a breaking point in the regulation of medical devices forcing the manufactures to investigate the overall qualitative composition of devices made of substances and quantitative information for the main constituents or for the components responsible for the desired effect. In view of this stricter standardization of plant-based medical devices, an intense research towards advanced technologies and robust analytical protocols is required. Based on these considerations, Grintuss® syrup was selected as a case study of formulated natural complex products for quali-quantitative metabolomics investigations. In particular, in the first part, an untargeted metabolomic approach for monitoring the batch-quality of Grintuss® syrup by electrospray ionization mass spectrometry flow injection analysis was developed. After having assessed the validity of the model by using multivariate statistical process control, the quality verification of new batches under investigation was achieved by comparing their profile with the fingerprinting profile of batches prepared according to the optimized and validated manufacturing process. The second task was aimed at profiling the metabolites contained in Grintuss® syrup by applying a targeted metabolomic LC-MS-based approach. Thus, an in-house database of high-purity standard reference compounds was built. Then, after the acquisition of MS/MS spectra of standard compounds of interest, the matching between the detected metabolites from samples and standard reference compounds from the database was assessed. Finally, the quantitative analysis of the recognized metabolites was performed and the results thus achieved have been combined with information deriving from the analysis of additional compounds allowing to reach the knowledge of >99.9% of the overall composition of Grintuss® syrup.

Abstract
Tipologia del documento
Tesi di dottorato
Autore
Tamimi, Sara
Supervisore
Co-supervisore
Dottorato di ricerca
Ciclo
30
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
metabolomics, medical device
URN:NBN
DOI
10.6092/unibo/amsdottorato/8559
Data di discussione
17 Aprile 2018
URI

Altri metadati

Statistica sui download

Gestione del documento: Visualizza la tesi

^