Chemiluminescence DNA-based Nanotechnologies for Bioanalytical Applications

Marchegiani, Elisa (2021) Chemiluminescence DNA-based Nanotechnologies for Bioanalytical Applications, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Chimica, 33 Ciclo. DOI 10.48676/unibo/amsdottorato/9832.
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DNA as powerful building molecule, is widely used for the assembly of molecular structures and dynamic molecular devices with different potential applications, ranging from synthetic biology to diagnostics. The feature of sequence programmability, which makes it possible to predict how single stranded DNA molecules fold and interact with one another, allowed the development of spatiotemporally controlled nanostructures and the engineering of supramolecular devices. The first part of this thesis addresses the development of an integrated chemiluminescence (CL)-based lab-on-chip sensor for detection of Adenosine-5-triphosphate (ATP) life biomarker in extra-terrestrial environments.Subsequently, we investigated whether it is possible to study the interaction and the recognition between biomolecules and their targets, mimicking the intracellular environment in terms of crowding, confinement and compartmentalization. To this purpose, we developed a split G-quadruplex DNAzyme platform for the chemiluminescent and quantitative detection of antibodies based on antibody-induced co-localization proximity mechanism in which a split G-quadruplex DNAzyme is led to reassemble into the functional native G-quadruplex conformation as the effect of a guided spatial nanoconfinement.The following part of this thesis aims at developing chemiluminescent nanoparticles for bioimaging and photodynamic therapy applications.In chapter5 a realistic and accurate evaluation of the potentiality of electrochemistry and chemiluminescence (CL) for biosensors development (i.e., is it better to “measure an electron or a photon”?), has been achieved.In chapter 6 the emission anisotropy phenomenon for an emitting dipole bound to the interface between two media with different refractive index has been investigated for chemiluminescence detection.

Tipologia del documento
Tesi di dottorato
Marchegiani, Elisa
Dottorato di ricerca
Settore disciplinare
Settore concorsuale
Parole chiave
DNA Nanotechnology, Supramolecular Chemistry, Analatycal Chemistry, Biosensor, Chemiluminescence
Data di discussione
20 Maggio 2021

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