Effects of glyphosate and Roundup upon mammalian gametes

Nerozzi, Chiara (2021) Effects of glyphosate and Roundup upon mammalian gametes, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie, 33 Ciclo. DOI 10.48676/unibo/amsdottorato/9686.
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The wide use of glyphosate-based herbicides (GBHs) has become a controversial issue due to the potential harmful effects on human health. Commercial formulations, among which Roundup is the most famous one, contain a number of adjuvants inside; most of these are patented and not publicly known, therefore, they can act differently from glyphosate alone and might strengthen its toxic effect. Our study is focused on GBHs reproductive toxicity with a special regard to glyphosate and Roundup impact on male and female mammalian gametes after exposure to concentrations ranging from the one recommended for agricultural use (0.1% Roundup, containing 360 µg/mL glyphosate) to 70-fold lower or more. Sperm quality analysis, either on boar and stallion, showed that Roundup has much more detrimental impact than glyphosate at equivalent concentrations on spermatozoa function and survival. Basing on our results, the toxic effect of these pesticides on spermatozoa may be linked to an impairment in mitochondrial activity and a subsequent decrease in ATP production and/or alterations in the redox balance, which impact cell motility and plasma membrane stability. Moreover, a different species sensitivity to GBHs may exists as high doses of glyphosate affected sperm quality only in boar and not in stallion; furthermore, Roundup had deleterious effects at lower doses in the first compared to the latter. With regard to female gametes, we found that glyphosate and Roundup exposure during IVM detrimentally affect the subsequent developmental ability of swine embryos, providing further evidence of their potential toxic effect on female reproductive system. In addition, Roundup altered steroidogenesis and increased oocyte ROS levels. Therefore, according to our results, we can conclude that GBHs exert a negative impact on both male and female gametes and that Roundup adjuvants enhance glyphosate toxic effects and/or are biologically active in their side-effect.

Tipologia del documento
Tesi di dottorato
Nerozzi, Chiara
Dottorato di ricerca
Settore disciplinare
Settore concorsuale
Parole chiave
glyphosate, Roundup, toxicity, reproduction, mammalian gametes, swine, stallion, sperm quality, CASA system, sperm motility, flow cytometry, viability, acrosome reaction, mitochondrial activity, SCSA, oocytes maturation, fertility, steroidogenesis, GSH, ROS
Data di discussione
19 Maggio 2021

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