Physical Mutagenesis in Giant Reed (Arundo Donax L.) and Phenotypic and Genomic Characterization of Mutagenized Clones

Valli, Fabio (2017) Physical Mutagenesis in Giant Reed (Arundo Donax L.) and Phenotypic and Genomic Characterization of Mutagenized Clones, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze e tecnologie agrarie, ambientali e alimentari, 28 Ciclo. DOI 10.6092/unibo/amsdottorato/8196.
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ABSTRACT Giant reed (Arundo donaxL.) is a C3 perennial, warm-season, rhizomatous grass of emerging interest for bioenergy and biomass-derivatives production, and for phytoremediation. We developed and applied a mutagenesis protocol based on γ-irradiation of in-vitro cell cultures from which regenerants were obtained. Based on a radio-sensitivity test, the irradiation dose reducing to 50% the number of regenerants per callus (RD50) was estimated at 35 Gy. A large mutagenic experiment was carried out by irradiating a total of 3,120 calli with approx. 1x, 1.5x and 2x RD50. A total of 1,004 regenerants from irradiated calli were hardened in pots and transplanted to the field. Approx. 10% of field grown clones showed remarkable morphological aberrations including dwarfism, altered tillering, abnormal inflorescence, leaf variegation and others, which were tested for stability over generations. Clone lethality reached 0.4%. Our results show for the first time that physical mutagenesis can efficiently induce new genetic and phenotypic variation of agronomic and prospective industrial value in giant reed. 100 clones of A. donax were chemically analyzed for several key chemical components of plant biomass including lignin, cellulose, hemicelluloses, non-structural carbohydrates, ash, and others. The complete chloroplast genome of A. donax is 139353 bp in length subdivide in two inverted repeat region (IRa and IRb) of 22,227 bp each separated by a small-single-copy-region of of 12,275 bp (SSC) and a large-single-copy-region of 82,124 bp (LSC). The genome includes 112 individual genes including 72 protein coding genes, 30 tRNA, 6 rRNA, 3 open reading frames and one pseudogene. FISH ang GISH analysis were performed on three species A. donax, A. plinii and P. australis in order to define the genetic structure of these species and the phylogenetic relationship existing among them.

Tipologia del documento
Tesi di dottorato
Valli, Fabio
Dottorato di ricerca
Scuola di dottorato
Scienze agrarie
Settore disciplinare
Settore concorsuale
Parole chiave
bioenergy crop, biomass, feedstock, gamma-ray, genetic diversity, marginal land, perennial grass, polyploidy, chloroplast genome, phylogenesis, chemical analysis, FISH (fluorescent in situ hybridization, GISH (genomic in situ hybridization)
Data di discussione
23 Maggio 2017

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