Valutazione dell'attività delle metalloproteinasi 2 e 9 nelle urine e nel tessuto renale di cani normali e affetti da nefropatia

Mancini, Danilo (2008) Valutazione dell'attività delle metalloproteinasi 2 e 9 nelle urine e nel tessuto renale di cani normali e affetti da nefropatia, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Diagnostica collaterale in medicina interna veterinaria, 19 Ciclo. DOI 10.6092/unibo/amsdottorato/737.
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Abstract

Matrix metalloproteinases (MMP) are a large family of proteinases that remodel extracellular matrix (ECM) component. Recent data suggest a role for MMPs in a number of renal pathophysiologies, associated with an imbalance of ECM syntesis and degradation, which may result in an accumulation of ECM molecules and renal fibrosis. The aim of this study is to elucidate the role of pro and activated MMP-2 and 9 in urine and renal tissue of healty and nephropatic dogs. Renal tissue of 8 healty dogs and either renal tissue and urine of 9 nephropatic dogs was collected and analize using zimographic method, which is been validated in this study. Either MMPs zimographic bands were present in almost all samples. In particular, pro and activated MMP-9 zimographic bands were poorly represent in renal tissue of healty dogs, whereas were very represent in nephropatic dogs. Pro and activated MMP-2 was present in either tissue of healty and nephropatic dogs. In urine of nephropatic dogs, pro and activated MMP-9 was more evident than MMP-2, but there was not correlaction with renal tissue levels, therefore urine levels of MMPs have poorly usefulness in diagnostic pratice. The values of Pro and activated MMP-9 in nephropatic dogs were significantly higher compared with normal dogs (p < 0,05), whereas there was not statistically meaningful for Pro and activated MMP-2. In conclusion, in this study we have validated a zimographic method for renal tissue of dogs and we have illustrated the changes in nephropatic dogs, which may be useful for further study.

Abstract
Tipologia del documento
Tesi di dottorato
Autore
Mancini, Danilo
Supervisore
Dottorato di ricerca
Ciclo
19
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
metalloproteinasi nefropatia fibrosi matrice extracellulare
URN:NBN
DOI
10.6092/unibo/amsdottorato/737
Data di discussione
11 Aprile 2008
URI

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