Closed-loop investigation of the dynamical properties of cardiomyocyte electrophysiology by means of Dynamic clamp and Mathematical modelling

Bartolucci, Chiara (2015) Closed-loop investigation of the dynamical properties of cardiomyocyte electrophysiology by means of Dynamic clamp and Mathematical modelling, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Bioingegneria, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/7163.
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Abstract

The research field of my PhD concerns mathematical modeling and numerical simulation, applied to the cardiac electrophysiology analysis at a single cell level. This is possible thanks to the development of mathematical descriptions of single cellular components, ionic channels, pumps, exchangers and subcellular compartments. Due to the difficulties of vivo experiments on human cells, most of the measurements are acquired in vitro using animal models (e.g. guinea pig, dog, rabbit). Moreover, to study the cardiac action potential and all its features, it is necessary to acquire more specific knowledge about single ionic currents that contribute to the cardiac activity. Electrophysiological models of the heart have become very accurate in recent years giving rise to extremely complicated systems of differential equations. Although describing the behavior of cardiac cells quite well, the models are computationally demanding for numerical simulations and are very difficult to analyze from a mathematical (dynamical-systems) viewpoint. Simplified mathematical models that capture the underlying dynamics to a certain extent are therefore frequently used. The results presented in this thesis have confirmed that a close integration of computational modeling and experimental recordings in real myocytes, as performed by dynamic clamp, is a useful tool in enhancing our understanding of various components of normal cardiac electrophysiology, but also arrhythmogenic mechanisms in a pathological condition, especially when fully integrated with experimental data.

Abstract
Tipologia del documento
Tesi di dottorato
Autore
Bartolucci, Chiara
Supervisore
Dottorato di ricerca
Scuola di dottorato
Scienze e ingegneria dell'informazione
Ciclo
27
Coordinatore
Settore disciplinare
Settore concorsuale
Parole chiave
Computational Biology, Cardiac Electrophysiology, Dynamic Clamp Cardiac Electrophysiology Dynamic Clamp
URN:NBN
DOI
10.6092/unibo/amsdottorato/7163
Data di discussione
8 Maggio 2015
URI

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