Drug of abuse analysis: new methods in herbal and biological matrices for medicinal chemistry and forensic investigation

Cirrincione, Marco (2023) Drug of abuse analysis: new methods in herbal and biological matrices for medicinal chemistry and forensic investigation, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Salute, sicurezza e sistemi del verde, 35 Ciclo.
Documenti full-text disponibili:
[img] Documento PDF (English) - Accesso riservato fino a 17 Gennaio 2026 - Richiede un lettore di PDF come Xpdf o Adobe Acrobat Reader
Disponibile con Licenza: Creative Commons Attribution Non-commercial No Derivatives 4.0 (CC BY-NC-ND 4.0) .
Download (3MB) | Contatta l'autore


In this Ph.D. project, original and innovative approaches for the quali-quantitative analysis of abuse substances, as well as therapeutic agents with abuse potential and related compounds were designed, developed and validated for application to different fields such as forensics, clinical and pharmaceutical. All the parameters involved in the developed analytical workflows were properly and accurately optimised, from sample collection to sample pretreatment up to the instrumental analysis. Advanced dried blood microsampling technologies have been developed, able of bringing several advantages to the method as a whole, such as significant reduction of solvent use, feasible storage and transportation conditions and enhancement of analyte stability. At the same time, the use of capillary blood allows to increase subject compliance and overall method applicability by exploiting such innovative technologies. Both biological and non-biological samples involved in this project were subjected to optimised pretreatment techniques developed ad-hoc for each target analyte, making also use of advanced microextraction techniques. Finally, original and advanced instrumental analytical methods have been developed based on high and ultra-high performance liquid chromatography (HPLC,UHPLC) coupled to different detection means (mainly mass spectrometry, but also electrochemical, and spectrophotometric detection for screening purpose), and on attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) for solid-state analysis. Each method has been designed to obtain highly selective, sensitive yet sustainable systems and has been validated according to international guidelines. All the methods developed herein proved to be suitable for the analysis of the compounds under investigation and may be useful tools in medicinal chemistry, pharmaceutical analysis, within clinical studies and forensic investigations.

Tipologia del documento
Tesi di dottorato
Cirrincione, Marco
Dottorato di ricerca
Settore disciplinare
Settore concorsuale
Parole chiave
Medicinal Chemistry; Analytical methods; Method development and validation; Sample pretreatment; Abuse drugs; Pharmaco-toxicological analysis.
Data di discussione
27 Marzo 2023

Altri metadati

Gestione del documento: Visualizza la tesi